Brites, Dora


Universidad o Centro de Investigación: Faculdade de Farmacia da Universidade de Lisboa  

Departamento, unidad o laboratorio: Instituto de Investigação do Medicamento e das Ciências Farmacêuticas  

Dirección postal: Faculdade de Farmacia da Universidade de Lisboa
Av. Prof. Gama Pinto
1649-003 Lisbon
Portugal
 

Código postal: 1649-003 Lisboa  

País: Portugal  

Teléfonos de contacto:  

+351217946450  

Fax: 353217946491  

Correos electrónicos de contacto:  

 dbrites@ff.ul.pt 


Linea(s) de investigación

• Uncovering the potential neurodevelopmental deficits in common neonatal conditions such as hyperbilirubinemia and inflammation
• Study of the blood-brain barrier disruption by neurotoxins and modulation by therapeutic agents in a human model: effects of bilirubin and endotoxin on brain microvascular endothelial cell monolayer integrity and protective role of bile acids
• Importância do perfil dos ácidos biliares séricos na avaliação da disfunção hepática”
• Unravelling the role of microglia and inflammation in bilirubin-induced neurologic dysfunction (BIND): from in vitro and ex vivo studies to BIND animal models
• From neural stem/progenitor cells to glioma: dissecting the tumorigenic properties, cell migration, angiogenesis and potential adjuvant chemotherapy

Miembros del grupo

Nombre: Rui Fernando Marques da Silva E-mail: rfmsilva@ff.ul.pt  

Nombre: Leonor Correia E-mail: mcorreia@ff.ul.pt  

Nombre: Adelaide Fernandes E-mail: amaf@ff.ul.pt  

Nombre: Alexandra Brito E-mail: abrito@ff.ul.pt  

Nombre: Ana Sofia Falcão E-mail: asfalcao@ff.ul.pt  

Nombre: Ana Rita Vaz E-mail: armvaz@ff.ul.pt  

Nombre: Andreia Barateiro E-mail: apb@ff.ul.pt  

Nombre: Ema Torrado E-mail: ema.torrado@gmail.com  

Nombre: Inês Palmela E-mail: inespalmela@ff.ul.pt  

Nombre: Filipa Cardoso E-mail: filipacardoso@ff.ul.pt  

Nombre: Sandra Silva E-mail: sandrasilva@ff.ul.pt  

Publicaciones más relevantes

Brites D. Bilirubin Injury to Neurons and Glial Cells: New Players, Novel Targets and Newer Insights. Semin Perinatol 2011 (in press).
Fernandes A., Barateiro A., Falcão A.S., Silva, S. L., Vaz A.R., Brito M.A. Silva, R.F.M., Brites D. Astrocyte reactivity to unconjugated bilirubin requires TNF-alpha and IL-1beta receptor signaling pathways. Glia. 2011;59:14-25.
Silva SL, Vaz AR, Barateiro A, Falcão AS, Fernandes A, Brito MA, Silva RF, Brites D. Features of bilirubin-induced reactive microglia: From phagocytosis to inflammation. Neurobiol Dis. 2010;40:663-75.
Cardoso FL, Brites D, Brito MA. Looking at the blood-brain barrier: molecular anatomy and possible investigation approaches. Brain Res Rev. 2010; 64:328-63.
Bernas MJ, Cardoso FL, Daley SK, Weinand ME, Campos AR, Ferreira AJ, Hoying JB, Witte MH, Brites D, Persidsky Y, Ramirez SH, Brito MA. Establishment of primary cultures of human brain microvascular endothelial cells to provide an in vitro cellular model of the blood-brain barrier. Nat Protoc. 2010;5:1265-72.
Brito MA, Vaz AR, Silva SL, Falcão AS, Fernandes A, Silva RF, Brites D. N-methyl-D-aspartate receptor and neuronal nitric oxide synthase activation mediate bilirubin-induced neurotoxicity. Mol Med. 2010; 16:372-380.
Fernandes A and Brites D. Contribution of inflammatory processes to nerve cell toxicity by bilirubin and efficacy of potential therapeutic agents. Curr Pharml Design 2009; 15:2915-26.
Vaz AR, Delgado-Esteban M, Brito MA, Bolaños JP, Brites D, Almeida A. Bilirubin selectively inhibits cytochrome c oxidase activity and induces apoptosis in immature cortical neurons. Assessment of the protective effects of glycoursodeoxycholic acid. J Neurochem 2010 112:56-65.
Fernandes A, Falcão AS, Abranches E, Bekman E, Henrique D, Lanier LM, Brites D. Bilirubin as a determinant for altered neurogenesis, neuritogenesis, and synaptogenesis. Dev Neurobiol. 2009;69:568-82.
Brites D, Fernandes A, Falcão AS, Gordo AC, Silva RFM, Brito MA. Biological risks for neurological abnormalities associated with hyperbilirubinemia. J Perinatol. 2009;29 Suppl 1:S8-13.
Costa E, Vieira E, Lopes AL, Saldanha MJ, Brites D, Santos R. Identification of a novel deletion in UDP-glucuronosyltransferase gene in a patient with Crigler-Najjar syndrome type I. Blood Cells Mol Dis. 2009;42:265-6.
Popko J, Fernandes A, Brites D, Lanier LM. Automated analysis of NeuronJ tracing data. Cytometry A. 2009;75:371-6.
Brites D, Fernandes A, Falcão AS, Brito MA, Silva RFM.Glial and Neuronal Reactivity to Unconjugated Bilirubin. In: Binder MD, Hirokawa N, Windhorst U, Hirsch MC (eds) Encyclopedia of Neuroscience. Neuroimmunology. Springer, Heidelberg, Germany, 2009, pp. 1726-1730.

Técnicas y modelos

In vitro model of human blood brain barrier
Primary cultures of astrocytes, microglia, neurons, olygodendrocytes and human brain microvascular endothelial cells
Co-cultures
Organotypic slice cultures
TEER
Evaluation of neurite extension and ramification
Synaptogenesis
Tight and adherens junction detection
neurospheres
gliogenesis and neurogenesis
inflammatory signaling pathways
Immunofluorescence
Western Blot
RT-PCR
Cell death (necrosis, apoptosis)
RE stress
cell redox status

Organización de Seminarios y Eventos

- Cultura de células cerebrais - Noções básicas/Implementação Curso Pós-graduado de Actualização
2010 - 25 a 29 de Janeiro
- Actualização às Metodologias de Avaliação da Inflamação Workshop - 2009 - 19 a 21 de Fevereiro
- Players in Brain Deregulation (Scientific Meeting)- 2010
Session I Microglia
13-14 December
Session II Neurons
20-21 December

Convenios con Empresas Privadas o Laboratorios

Objetivos para los próximos años

Current research goals are to further explore determinants and modifiers of neuronal and glial differentiation and dedifferentiation, neuritic arborization, nerve cell reactivity and fate during CNS injury and repair, as well as of BBB biology and modulation. Ultimately, the group fosters to assess the efficacy/toxicity of new preventive and therapeutic strategies, as well as novel delivery approaches to CNS disorders.

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